Size Exclusion Chromatography (SEC)

Size Exclusion Chromatography (SEC) separates molecules based on their size, or more precisely, their hydrodynamic volume. It is based on the discrimination of individual sample components by the pores of the packing material. Large sample molecules cannot or can only partially penetrate the pores and elute from the column first, whereas smaller molecules can access all or a larger number of pores and elute later. SEC is the only mode of chromatography that does not involve interaction with a stationary phase by means of adsorption or partitioning of the solutes.

Avantor® Hichrom other columns

Eprogen, Exsil, Avantor® Hichrom C8, C18 and RPB, Avantor® Hichrom PAH2, Avantor® Hichrom Chiral, Inertsil, LiChrosorb, LiChrospher, NUCLEOSIL, Avantor® Ultrasphere, ZORBAX

HPLC columns, Avantor® Partisil® & Partisphere®

Commercially available spherical silicas and it continues to provide and reproducible, high efficiency separations, Avantor® Partisphere® columns are available in a wide range of surface chemistries including two application specific phases.

Stationary Phases Acquired from Grace

Avantor® are pleased to announce we have acquired the worldwide exclusive rights to manufacture Vydac®, Alltima®, Alltima® HP, Prevail™, Apollo™, Allsep®, Apex™ and Genesis™ analytical HPLC column ranges from Grace/Alltech. Avantor® have now commenced manufacture of these columns to the same exacting manufacturing protocols and to identical specifications previously used by Grace/Alltech. Part numbers also remain unaffected by the acquisition.

Avantor® ACE® HPLC and UHPLC Columns

Avantor® manufacture the ultra-inert Avantor® ACE® UHPLC/HPLC column range. Avantor® ACE® phases incorporate the latest developments in LC stationary phase design, providing chromatographers with more choices for alternative selectivity, without compromising stability or robustness. Avantor® ACE® LC phases have been specifically designed to help solve chromatographic challenges and include the unique C18-AR, C18-PFP, C18-Amide, CN-ES, SuperC18 and SuperPhenylHexyl phases, as well as a wide range of other 100 Å and 300 Å phases. Discover how Avantor® ACE® selectivities can help you with your chromatographic challenges. Avantor® ACE® – superior LC columns.

MonoCap High Resolution

MonoCap C18 HighResolution 2000 is a 2-meter length monolithic silica capillary column which is designed for identifying extremely high number of peptides/proteins for proteome research via LC-MS/MS.
MonoCap HILIC-UP is an important addition to the MonoCap C18 HighResolution 2000 column series. MonoCap HILIC-UP can retain highly hydrophilic peptides/proteins which may lead to discovering new peptides/proteins where a C18 phase couldn’t identify. In HILIC, the higher the organic concentration, the greater the retention of more polar analytes. One of the biggest benefits of HILIC mode is, a high organic solvent concentration of the mobile phase will lead to a high sensitivity LC-MS/MS analysis.

InertSustain HPLC Columns

Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties. InertSustain inherits the advantages of all the current Inertsil HPLC columns (e.g., extremely low operating back pressure, superior inertness to typically any analytes, high efficiency and compatibility with a wide range of solvents), but now can be used for wide pH analysis with consistent performance from column to column and lot to lot.
The InertSustain Serie has the following columns available; InertSustain C18, InertSustain AQ-C18, InertSustainSwift C18, InertSustainSwift C8, InertSustain Phenyl, InertSustain Phenylhexyl, InertSustain Cyano, InertSustain NH2, InertSustain Amide, InertSustainBio C18.

Inertsil HPLC Columns

GL Sciences in Japan is the manufacturer of well-known Inertsil HPLC columns. GL Sciences’ Inertsil columns offer everything you need for your critical separations in all phases of drug development, and something less. Inertsil’s uniquely modified silicas and meticulously engineered column hardware allow our columns to operate at significantly lower back pressures than competitive columns, without sacrificing efficiency. Inertsil’s wide pH compatibility and excellent stability at elevated temperatures make it the ideal choice for your chromatography needs, whether you are using a traditional HPLC system or the latest rapid gradient LC/MS system with precise temperature control.

Micro/NanoLC Columns

Miniaturisation of liquid chromatography in combination with mass spectrometry has several advantages including improvements in sensitivity, especially at low concentration levels and dramatically reduced solvent consumption, compared to conventional HPLC or UHPLC. With further method optimisation, run times can also be reduced giving further savings in solvent use or time.
To meet the requirements of MicroLC/CapillaryLC/NanoLC YMC offers capillary columns specifically designed to use with the corresponding chromatography systems.

Normal Phase (NP) Columns

Whilst historically it was the earliest form of HPLC, normal phase separations have recently received less attention due to the belief that it is complicated and unpredictable. However, normal phase chromatography is a powerful tool for the separation of positional isomers that are difficult to separate in reversed phase mode. Due to a rigid surface, compared to the more flexible carbon chains of reversed phases, the analytes are influenced by well-defined steric interaction with polar groups. YMC offers columns packed with non-bonded silica or with silica gel modified with polar groups

HILIC Columns

HILIC HPLC/UHPLC columns from YMC are rugged stationary phases which provide improved LC/ESI-MS response, direct SPE solvent compatibility and complementary selectivity to reversed phases. This is important to R&D and drug metabolism scientists since the impurity or metabolite is frequently more polar and present at much lower concentrations than the parent compound. With YMC HILIC columns, these very polar compounds elute later than the higher hydrophobic parent compound, thereby minimizing the MS ion suppression that can occur at the beginning of the chromatogram.

Chiral Columns

YMC offers several solutions for separating chiral compounds with different chiral selectors and different chiral separation mechanisms. The selectors are either coated/immobilised or bonded to the support material which has different pore sizes dependent on the selector. According to the selector chiral chromatography can be performed in NP, RP and/or SFC mode.