Στήλες HPLC & UHPLC

Inertsil HPLC Columns

GL Sciences in Japan is the manufacturer of well-known Inertsil HPLC columns. GL Sciences’ Inertsil columns offer everything you need for your critical separations in all phases of drug development, and something less. Inertsil’s uniquely modified silicas and meticulously engineered column hardware allow our columns to operate at significantly lower back pressures than competitive columns, without sacrificing efficiency. Inertsil’s wide pH compatibility and excellent stability at elevated temperatures make it the ideal choice for your chromatography needs, whether you are using a traditional HPLC system or the latest rapid gradient LC/MS system with precise temperature control.

SFC Columns

YMC offers chiral and achiral phases for supercritical fluid chromatography (SFC) applications to provide the chemist with a number of options for SFC separations. SFC can offer some advantages over HPLC for similar applications. The low viscosity of supercritical carbon dioxide allows for analytical separations 3-5 times faster than those for normal phase HPLC. Speed of the SFC separations, conservation of organic solvents, and more concentrated product fractions make SFC a desirable preparative chromatographic technique for purifying chemical mixtures. YMC’s columns are 100% compatible with SFC conditions. Further, Alcyon SFC columns are available, which are specifically packed in a SFC compatible hardware and are tested under SFC conditions.

BioPro HIC HT

HIC Columns for High Throughput Analyses
Ideal drug-to-antibody ratio (DAR) analysis due to novel surface chemistry
High throughput by reducing analysis time
Excellent batch-to-batch reproducibility

Biocompatible YMC-Triart metal-free

Inert columns for bio separations and coordinating compounds
Excellent peak shapes and high sensitivity
Reproducible results day-after-day
No carry-over effects
Ideal for highly sensitive LC/MS analyses
High pressure limits
Ideal choice for Sensitive proteins or peptides, Nucleotides, oligonucleotides, Metal coordinating compounds

Reprospher

Your workhorse
Reprospher media are based on a 100 A ultrapure silica and are fully scalable from UPLC (1.9 µm particles) to preparative and process scale applications.
Among the offered phases there are some unique selectivities such as a wide range of aromatic specialty phases, phases with polar selectivities and unique phases for achiral SFC.

ReproSil XR

Premium performance at exceptional value
An allround and fully scalable spherical Silica from 1.5 to 15 µm with an extra high purity.
The metal content is less than 100 ppb.
The silica impresses with its very narrow pore size distribution this make 99 % of the surface accessible for the separation.
The uniform bonded phase coverage translates to symmetrical peaks for acids/bases, and predictable reversed phase selectivity.
Because of its very low metal content it also impressed with a high pH stability.

ReproSil Saphir

Alternative to Luna C18
A low metal content spherical silica media scalable from sub-2-micron to 10 µm particles with high carbon load for small molecules and peptides and wide pore ion exchange media for protein and enzyme analysis and purification.

ReproSil-Pur

Specifically designed for pharmaceutical and biotechnical separations.
A robust modern and all-purpose ultra-high purity spherical silica media scalable from UPLC to prep.
Various pore sizes are available to cover applications for small molecules up to biomolecules.
State-of-the-art surface modification technologies are employed for normal and reversed phase media including shape selective, high carbon load as well as water wettable alkyl phases.

Micro/NanoLC Columns

Miniaturisation of liquid chromatography in combination with mass spectrometry has several advantages including improvements in sensitivity, especially at low concentration levels and dramatically reduced solvent consumption, compared to conventional HPLC or UHPLC. With further method optimisation, run times can also be reduced giving further savings in solvent use or time. To meet the requirements of MicroLC/CapillaryLC/NanoLC YMC offers capillary columns specifically designed to use with the corresponding chromatography systems.

Normal Phase (NP) Columns

Whilst historically it was the earliest form of HPLC, normal phase separations have recently received less attention due to the belief that it is complicated and unpredictable. However, normal phase chromatography is a powerful tool for the separation of positional isomers that are difficult to separate in reversed phase mode. Due to a rigid surface, compared to the more flexible carbon chains of reversed phases, the analytes are influenced by well-defined steric interaction with polar groups. YMC offers columns packed with non-bonded silica or with silica gel modified with polar groups

HILIC Columns

HILIC HPLC/UHPLC columns from YMC are rugged stationary phases which provide improved LC/ESI-MS response, direct SPE solvent compatibility and complementary selectivity to reversed phases. This is important to R&D and drug metabolism scientists since the impurity or metabolite is frequently more polar and present at much lower concentrations than the parent compound. With YMC HILIC columns, these very polar compounds elute later than the higher hydrophobic parent compound, thereby minimizing the MS ion suppression that can occur at the beginning of the chromatogram.

Chiral Columns

YMC offers several solutions for separating chiral compounds with different chiral selectors and different chiral separation mechanisms. The selectors are either coated/immobilised or bonded to the support material which has different pore sizes dependent on the selector. According to the selector chiral chromatography can be performed in NP, RP and/or SFC mode.

Biochromatography Columns

RP Bioseparation,
Ion Exchange (IEX),
Size Exclusion (SEC),
Hydrophobic Interaction (HIC)

Reversed Phase (RP) Columns

YMC’s selection of reversed phase (RP) UHPLC/HPLC columns
YMC-Triart,
YMC-Pack ProFamily,
YMC RP Classics,
Meteoric Core

Application Specific HPLC Columns

Designed for key pharmaceutical and environmental applications, these columns utilize novel and unique chemistries to provide superior resolution with ease of use.

Size Exclusion HPLC Columns

Want high resolution separation of water-soluble polymers? Our size exclusion columns (SEC) are available in tailored pore sizes to cover a broad range of molecular weight.