{"id":1470,"date":"2026-04-03T11:06:12","date_gmt":"2026-04-03T08:06:12","guid":{"rendered":"https:\/\/metrolab.cen.aboutdev.gr\/?p=1470"},"modified":"2026-04-30T13:29:41","modified_gmt":"2026-04-30T10:29:41","slug":"normal-phase-np-columns","status":"publish","type":"post","link":"https:\/\/metrolab.gr\/en\/normal-phase-np-columns\/","title":{"rendered":"Normal Phase (NP) Columns"},"content":{"rendered":"

Whilst historically it was the earliest form of HPLC, normal phase separations have recently received less attention due to the belief that it is complicated and unpredictable. However, normal phase chromatography is a powerful tool for the separation of positional isomers that are difficult to separate in reversed phase mode. Due to a rigid surface, compared to the more flexible carbon chains of reversed phases, the analytes are influenced by well-defined steric interaction with polar groups. YMC offers columns packed with non-bonded silica or with silica gel modified with polar groups<\/p>","protected":false},"excerpt":{"rendered":"

Whilst historically it was the earliest form of HPLC, normal phase separations have recently received less attention due to the belief that it is complicated and unpredictable. However, normal phase chromatography is a powerful tool for the separation of positional isomers that are difficult to separate in reversed phase mode. Due to a rigid surface, compared to the more flexible carbon chains of reversed phases, the analytes are influenced by well-defined steric interaction with polar groups. YMC offers columns packed with non-bonded silica or with silica gel modified with polar groups<\/p>","protected":false},"author":2,"featured_media":1456,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[57],"tags":[109],"class_list":["post-1470","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-ymc","tag-category"],"acf":[],"yoast_head":"\nNormal Phase (NP) Columns - MetroLab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/metrolab.gr\/en\/normal-phase-np-columns\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Normal Phase (NP) Columns - MetroLab\" \/>\n<meta property=\"og:description\" content=\"Whilst historically it was the earliest form of HPLC, normal phase separations have recently received less attention due to the belief that it is complicated and unpredictable. However, normal phase chromatography is a powerful tool for the separation of positional isomers that are difficult to separate in reversed phase mode. Due to a rigid surface, compared to the more flexible carbon chains of reversed phases, the analytes are influenced by well-defined steric interaction with polar groups. 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