1.0MetroLabhttps://metrolab.gr/enpraktikihttps://metrolab.gr/en/author/praktiki/rich600338<blockquote class="wp-embedded-content" data-secret="f6pkGfyyYx"><a href="https://metrolab.gr/en/reversed-phase-chromatography-rplc-or-rpc/">Reversed Phase Chromatography (RPLC or RPC)</a></blockquote><iframe sandbox="allow-scripts" security="restricted" src="https://metrolab.gr/en/reversed-phase-chromatography-rplc-or-rpc/embed/#?secret=f6pkGfyyYx" width="600" height="338" title="“Reversed Phase Chromatography (RPLC or RPC)” — MetroLab" data-secret="f6pkGfyyYx" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" class="wp-embedded-content"></iframe><script> /*! This file is auto-generated */ !function(d,l){"use strict";l.querySelector&&d.addEventListener&&"undefined"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[^a-zA-Z0-9]/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret="'+t.secret+'"]'),o=l.querySelectorAll('blockquote[data-secret="'+t.secret+'"]'),c=new RegExp("^https?:$","i"),i=0;i<o.length;i++)o[i].style.display="none";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute("style"),"height"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener("message",d.wp.receiveEmbedMessage,!1),l.addEventListener("DOMContentLoaded",function(){for(var e,t,s=l.querySelectorAll("iframe.wp-embedded-content"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute("data-secret"))||(t=Math.random().toString(36).substring(2,12),e.src+="#?secret="+t,e.setAttribute("data-secret",t)),e.contentWindow.postMessage({message:"ready",secret:t},"*")},!1)))}(window,document); //# sourceURL=https://metrolab.gr/wp-includes/js/wp-embed.min.js </script>https://metrolab.gr/wp-content/uploads/2026/04/TOSOH_05.-Reversed-phase_2021.jpg300300Reversed Phase Chromatography (RPLC or RPC) is the most efficient of all biomolecule separation techniques. It has been the technique of choice for the analysis of small molar mass compounds in both the pharmaceutical and chemical industries, as well as in biomedical research, since the late 1970s. More recently, RPC has become the accepted tool for the separation of peptides, proteins and other biopolymers, making it largely responsible for the widespread popularity of HPLC as a chromatographic technique.